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The complex 7 exhibits an intense 1 MLCT absorption band at nm and luminesces at nm with a luminescence quantum yield of 3. The fluorescence microscopy imaging study of HeLa cells incubated with 7 and stained with ethidium bromide and acridine orange confirms that the cells are viable throughout the imaging experiments and its cytotoxicity against HeLa cells, studied by the MTT assay, demonstrates its use for bioimaging studies. Furthermore, apoptosis of HeLa cells, studied by the DNA ladder assay, indicates apoptotic cell death lending support for the antitumor activity of 7. A molecular docking study reveals that the complex 7 intercalates into the major groove of the DNA stabilized by hydrogen bonding and it binds with HSA by electrostatic- and hydrogen bonding interactions. The relaxometry, luminescence and fluorescence imaging studies indicate that the Ru II —Gd III 2 complex 7 has a good cell membrane permeability and could be considered as a potential bimodal imaging probe. The article was received on 04 Jun , accepted on 03 Sep and first published on 07 Sep If you are not the author of this article and you wish to reproduce material from it in a third party non-RSC publication you must formally request permission using Copyright Clearance Center.
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Salmonellosis, a communicable disease caused by members of the Salmonella species, transmitted to humans through contaminated food or water. It is of paramount importance, to generate accurate detection methods for discriminating the various Salmonella species that cause severe infection in humans, including S. Typhi and S. Paratyphi A. Here, we formulated a strategy of detection and differentiation of salmonellosis by a multiplex polymerase chain reaction assay using S.
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We got a furry little baby in June last year. We named him Bruno. He is a cuddly little golden retriever. He was my joy. He was my lucky charm. August got me realizing that I was pregnant. For a moment I thought it was a little horse galloping inside me.